Map showing sampling locations in the Muriaé Ornamental Aquaculture Center. Sampling sites: BV Boa Vista, BS Boa Sorte, GR Glória reservoir, RO Rochedo, PI Pinheiros, VA Varginha, SL São Luiz, CH Chato. Main municipalities of this aquarium fish center: Muriaé, Miradouro, Vieiras, São Francisco do Glória (Modified from Santana et al. 2010). Adult non-native species: A Nannostomus beckfordi (3.7 cm total length), B Aphyocharax anisitsi (4.9 cm total length), C Knodus moenkhausii (4.2 cm total length), D Moenkhausia costae (5.5 cm total length), E Ancistrus multispinis (9.8 cm total length), F Astronotus ocellatus (19.8 cm total length).